Rat anti PIG CD44:FITC 伯乐 MCA1449F CD45抗体

2024-10-29

Rat anti PIG CD44:FITC

货号:MCA1449F

规格:0.1 mg

市场价格:2950

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD44

物种:pig

宿主:大鼠

抗体亚型:IgG1

克隆号:MAC329

荧光染料:FITC

产品介绍
Rat anti Pig CD44 antibody, clone MAC329recognizes the porcine CD44 cell surface antigen which is involved in cell-cell interactions, cell adhesion and migration. CD44 is also a receptor for hyaluronic acid and can interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs).
产品详情

Target Species

Pig

Species Cross-Reactivity

Target Species

Cross Reactivity
Dog
Horse
Bovine
Goat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

1% Bovine Serum Albumin

ImmunogenPurified porcine CD44

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Fusion PartnersSpleen cells from immunised LOU Rats were fused with cells of the Y3.Ag1.2.3 myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood
同型
DescriptionProduct CodeApplicationsPack Size
Rat IgG1 Negative Control:FITCMCA6004FFlow0.1 mg
同型
DescriptionProduct CodeApplicationsPack Size
Mouse anti Pig CD3:FITCMCA5951FFlow0.1mg
文献
1.Yang, H.et al.(1993) Preparation and reactivities of anti-porcine CD44 monoclonal antibodies.Scand J Immunol. 37 (4): 490-8.2.Kasper, M.et al.(1996) Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs.Histochem Cell Biol. 105 (3): 221-30.3.Gyongyosi, M.et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.Thromb Haemost. 103: 1-9.4.Smith, M.M.et al.(2008) Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy.Rheumatology (Oxford). 47:1172-8.5.Sanchez, M.A.et al.(2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.Am J Trop Med Hyg. 70: 618-24.6.Lacroux, C.et al.(2012) Study of canine cutaneous melanocytic tumours: evaluation of histological and immunohistochemical prognostic criteria in 65 casesRevue de Médecine Vétérinaire 163: 393-401.7.Zimmermann, C.E.et al.(2021) Characterization of porcine mesenchymal stromal cells and their proliferative and osteogenic potential in long-term culture.J Stem Cells Regen Med. 17 (2): 49-55.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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