Rat anti mouse CD117:PE 伯乐 MCA1365PE CD117抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse CD117:PE 伯乐 MCA1365PE CD117抗体

2024-10-29

Rat anti mouse CD117:PE

货号：MCA1365PE

规格：100 Tests

市场价格：4148

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD117

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b

克隆号： 2B8

荧光染料：PE

产品介绍
Rat anti Mouse CD117 antibody, clone 2B8recognizes mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.Rat anti Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice,CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein A.
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Immunogen	IL-3 dependent mast cells.
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Fusion Partners	Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
相关产品
Description	Product Code	Applications	Pack Size
Mouse Seroblock FcR	BUF041A	F	0.1 mg
Mouse Seroblock FcR	BUF041B	F	0.5 mg
数据
Figure A. Alexa647® conjugated rat anti mouse CD11b (MCA74A647) and RPE conjugated rat IgG2b isotype control (MCA6006PE). Figure B. Alexa647® conjugated rat anti mouse CD11b (MCA74A647) and RPE conjugated rat anti mouse CD117 (MCA1365PE). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).
文献
1. Fleming WHet al.(1993) Steel factor influences the distribution and activity of murine hematopoietic stem cellsin vivo.Proc Natl Acad Sci U S A. 90 (8): 3760-4.2. Rico-Vargas, S.A.et al.(1994) c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody.J Immunol. 152 (6): 2845-52.3. Manova, K.et al.(1992) c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons.J Neurosci. 12 (12): 4663-76.4. Maeda, H.et al.(1992) Requirement of c-kit for development of intestinal pacemaker system.Development. 116 (2): 369-75.5. Takagi, M.et al.(1992) Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor.J Immunol. 148 (11): 3446-53.6. de Vries, P.et al.(1992) Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.J Exp Med. 176 (6): 1503-9.

技术参数

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Rat anti mouse CD117:PE 伯乐 MCA1365PE CD117抗体

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