mouse anti Rat CD31:Low Endotoxin 伯乐 MCA1334EL CD31抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti Rat CD31:Low Endotoxin 伯乐 MCA1334EL CD31抗体

2024-10-29

mouse anti Rat CD31:Low Endotoxin

货号：MCA1334EL

规格：0.5 mg

市场价格：5143

产品类型：标签抗体

品牌：Bio-Rad

抗原：CD31

物种：大鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：TLD-3A12

荧光染料：Low Endotoxin

产品介绍
Mouse anti Rat CD31 antibody, clone TLD-3A12recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S.et al.2009).Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilsonet al.2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Macleanet al.2001)
产品详情
Target Species	Rat
Species Cross-Reactivity	Target Species	Cross Reactivity
Rhesus Monkey	√
Pig	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	None present
Carrier Free	Yes
Immunogen	Activated, Lewis rat derived microglial cells.
Approx. Protein Concentrations	IgG concentration 1 mg/ml
Endotoxin Level	<0.01 EU/ug
Fusion Partners	Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®488	495	519
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Store at -20oC onlyThis product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/50	1/100
ELISA	√
Immunofluorescence	√
Immunohistology - Frozen	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells .
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:Low Endotoxin	MCA1209EL	F	0.5 mg
文献
1. Aminzadeh, A.et al.(2020) Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.Cell J. 22 (Suppl 1): 38-48.2. Pedram, M.S.et al.(2010) Transplantation of a combination of autologous neural differentiated and undifferentiated mesenchymal stem cells into injured spinal cord of rats.Spinal Cord. 48 (6): 457-63.3. Sheu, J.J.et al.(2012) Combination of cilostazol and clopidogrel attenuates rat critical limb ischemia.J Transl Med. 10: 164.4.Costa, B.P.et al.(2018) Intestinal Epithelial Stem Cells: Distinct Behavior After Surgical Injury and Teduglutide Administration.J Invest Surg. 31 (3): 243-52.5. Pun, C.K.et al.(2021) Glycyrrhizin Attenuates Portal Hypertension and Collateral Shunting via Inhibition of Extrahepatic Angiogenesis in Cirrhotic Rats.Int J Mol Sci. 22(14):7662.6. Huang, H.C.et al.(2021) Matrix metalloproteinase-9 inhibition or deletion attenuates portal hypertension in rodents.J Cell Mol Med. 25 (21): 10073-87.7. Huang, H.C.et al.(2021) Microbiota transplants from feces or gut content attenuated portal hypertension and portosystemic collaterals in cirrhotic rats.Clin Sci (Lond). 135 (24): 2709-2728.8. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with SaprophyticStaphylococcus epidermidis..Biomedicines. 10 (5): 1021.

技术参数

产品应用 IHC;ELISA;IF;Flow;WB

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mouse anti Rat CD31:Low Endotoxin 伯乐 MCA1334EL CD31抗体

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