mouse anti human CD13 伯乐 MCA1270 CD13抗体

2024-10-29

mouse anti human CD13

货号:MCA1270

规格:0.2 mg

市场价格:3315

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD13

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号: WM15

荧光染料:Purified

产品介绍
Mouse anti Human CD13 antibody, clone WM15recognizes human CD13 also known as aminopeptidase N. CD13 is a single pass type II glycosylated integral membrane protein with a predicted molecular mass of ~110 kDa and an apparent molecular mass of ~150 kDa expressed by granulocytes, monocytes, fibroblasts, endothelial cells and by myeloid leukaemia cells (Bradstocket al.1985). CD13 acts as a major cell surface receptor for group 1 coronoviruses (Breslinet al.2003) which bind to a critical sequence encompassing amino acid residies 288-295 (Kolbet al.1997).CD13 functions as anaminopeptidaseenzyme, a metalloprotease present as both a membrane bound form and also a soluble aminopeptidase N.Mouse anti Human CD13, clone WM15 inhibits infection of cells by human coronavirus (Lachanceet al.1998) but not hepatitis C virus (Koutsoudakiset al.2006) and inhibits aminopeptidase N activity of the CD13 molecule (Asmunet al.1992).
产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity
Rhesus Monkey

N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Carrier FreeYes
ImmunogenHuman AML cells.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line

Regulatory

For reseamatchrch purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

1/501/100
ELISA
Immunohistology - Frozen
Immunoprecipitation
Western BlottingX
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood
数据

Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (MCA1270). Medium power

Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15(MCA1270)red in A. and Mouse anti CD21 antibody, clone LB21 (MCA1195) green in B. The merged image is in C with nuclei counterstained blue using DAPI. Medium power.
同型
DescriptionProduct CodeApplicationsPack Size

Mouse IgG1 Negative Control

MCA928

F

100 Tests

文献
1. Stolzing, A.et al.(2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.Mech Ageing Dev. 129: 163-73.2. Silk, K.M.et al.(2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012:172420.3. Negussie, A.H.et al.(2010) Synthesis and in vitro evaluation of cyclic NGR peptide targeted thermally sensitive liposome.J Control Release. 143: 265-73.4. Lassnig, C.et al.(2005) Development of a transgenic mouse model susceptible to human coronavirus 229E.Proc Natl Acad Sci U S A. 102 (23): 8275-80.5. Thielitz, A.et al.(2004) Identification of extra- and intracellular alanyl aminopeptidases as new targets to modulate keratinocyte growth and differentiation.Biochem Biophys Res Commun. 321 (4): 795-801.6. McCormack, E.et al.(2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.Blood. 121 (7): e34-42.7. Fiddler, C.A.et al.(2016) The Aminopeptidase CD13 Induces Homotypic Aggregation in Neutrophils and Impairs Collagen Invasion.PLoS One. 11 (7): e0160108.8. Chaturvedi, C.P.et al.(2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.Stem Cells Int. 2018: 6901761.

技术参数

产品应用 IHC;ELISA;Flow;IP

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