EveryBlot Blocking BufferBio-rad伯乐封闭液试剂 12010020

2024-07-25

产品描述

一瓶封闭缓冲液,500毫升。所有western印迹只需要5分钟的封闭

每个印迹-5分钟每个印迹阻断

五分钟的阻断缓冲液,适用于所有印迹。

EveryBlot阻断缓冲液为所有蛋白质印迹提供5分钟的阻断和较大灵敏度,无论检测方法如何。

Bio-rad伯乐封闭液试剂 12010020

功能和优点

减少非特异性抗体结合以减少背景,同时保持优异的敏感性

用于化学发光和荧光检测

不含磷酸盐缓冲液。非常适合与磷酸特异性抗体一起使用

5分钟内完成封闭

Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots

competitor-everyblot-5 minute everyblot blocking

A five minute blocking buffer for ALL western blots.

EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.

Features and Benefits

Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity

For both chemiluminescent and fluorescent detection

Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies

Complete blocking in 5 minutes

Bio-rad伯乐封闭液试剂 12010020

使用建议

对于小型印迹,使用至少10 ml用于阻断和抗体孵育步骤。对于中等大小的印迹,至少使用20毫升。

将块加入膜中,搅拌孵育5分钟

将第一抗体和第二抗体稀释在全强度块中,并在搅拌下孵育1小时

对于PVDF的荧光检测,将SDS添加到第二抗体溶液中的0.02%。

Recommended Protocol

For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.

Add block to the membrane and incubate for 5 minutes with agitation

Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation

For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.

1610374PRECISION PLUS STD DUAL COLOR双色预染蛋白markerBio-rad伯乐
1610394Precision Plus Protein Dual Color Standards 5 Pack5个包装的双色预染蛋白markerBio-rad伯乐
1610375PREC PLUS STD KALEIDOSCOPE蓝色预染蛋白markerBio-rad伯乐
1620177IMMUN-BLOT PVDF MEM,26CMX3.3MPVDF膜Bio-rad伯乐
1705060Clarity Western ECL Substrate, 200ml100ml装Clarity化学发光液Bio-rad伯乐
1705061Clarity Western ECL Substrate, 500ml250ml装Clarity化学发光液Bio-rad伯乐
1708890KIT,iSCRIPT cDNA SYNTHESIS,25RiScript™ cDNA合成试剂盒Bio-rad伯乐
1708891KIT,iSCRIPT cDNA SYNTHES,100RiScript™ cDNA合成试剂盒Bio-rad伯乐
TBS0201TUBE,STRIP-8,NATL 120 STRIPSPCR8联管,高位Bio-rad伯乐
TCS0801CAP STRIP-8,DOMED 130 STRIPSPCR8联管盖Bio-rad伯乐
TCS0803OPTICAL,8-CAP STRIP,FLAT (120)PCR8联管盖通用Bio-rad伯乐
TLS0801TUBE,STRIP-8,LO-PRO NAT 120/PKPCR8联管,低位Bio-rad伯乐
TLS0851TUBE,STRIP-8,LO-PRO WHT 120/PKPCR8联管,低位Bio-rad伯乐
HSP9601HSP-96,WHT/CLR 50/BXHard-Shell® 96孔全裙边PCR反应板,低位通明Bio-rad伯乐
HSP9655HSP-96,WHT/WHT 50/BXHard-Shell® 96孔全裙边PCR反应板,低位白色Bio-rad伯乐
MLP9601MULTIPLATE-96,NATL 25/BXMultiplate96孔无裙边PCR反应板,高位Bio-rad伯乐

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-28227.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价