Bio-Rad AHP1419 PCNA ,Rabbit anti Human PCNA antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1419 PCNA ,Rabbit anti Human PCNA antibody

2024-10-22

Rabbit anti Human PCNA antibody

货号：AHP1419

规格：50 µg

价格：7310

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：PCNA

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Human PCNA antibodyrecognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36 kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovicset al.2009).PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohistochemistry on paraffin sections using rabbit anti human PCNA antibody (Guninet al.2014).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Antiserum Preparation	Antiserum to human PCNA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations	IgG concentration 0.5 mg/ml
Immunogen	Synthetic peptide corresponding an internal region of human PCNA protein.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/2000	1/8000
Immunohistology - Paraffin¹	√		2.5ug/ml
Western Blotting	√	1/500	1/2000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. ¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Histology Positive Control Tissue Breast
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数据
Staining of formalin fixed,paraffin embedded human breast tissue section with Rabbit anti Human PCNA antibody (AHP1419).
文献
1. Gunin, A.G.et al.(2010) Age-Related Changes in Proliferation, the Numbers of Mast Cells, Eosinophils, and cd45-Positive Cells in Human DermisJ Gerontol A Biol Sci Med Sci. 66: 385-92.2. Kim, J.Y.et al.(2012) Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats.Angle Orthod. 82: 107-14.3. Petrov. V.V.et al.(2013) Age-related changes in mast cells and eosinophils of human dermisOntogenez. 44: 179-85.4. Gunin, A.G.et al.(2014) Age-related changes in angiogenesis in human dermis.Exp Gerontol. 55C: 143-51.5. Di-Poi,N. & Milinkovitch, M. C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotesScience Advances. 2 (6): e1600708.

技术参数

产品应用 ELISA;IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;IHC(P)*;WB

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Bio-Rad AHP1419 PCNA ,Rabbit anti Human PCNA antibody

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