| 产品介绍 | | |
| Mouse anti Human CD284 antibody, clone HTA125recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL) is recommended for functional assays. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Alexa Fluor 488 - liquid |
| Preparation | - MCA2061A488: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA2061A488T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
|
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Ba/F3 cell line expressing TLR4 (CD284). |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 - Ag8 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control:Alexa Fluor® 488 | MCA929A488 | F | 100 Tests/1ml |
| 文献 |
| 1.Foster, N.et al.(2007) Pivotal advance: vasoactive intestinal peptide inhibits up-regulation of human monocyte TLR2 and TLR4 by LPS and differentiation of monocytes to macrophages.J Leukoc Biol. 81: 893-903.2.Shimazu, R.et al.(1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4.J Exp Med. 189 (11): 1777-82.3.Jiang, Q.et al.(2000) Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B.J Immunol. 165 (7): 3541-4.4.Yang, S.et al.(2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture.Infect Immun. 69 (4): 2045-53. |
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