BIO-RAD MCA2059T Mouse anti Human CD88 antibody, clone P12/1,Mouse anti Human CD88 antibody, clone P12/1

2024-10-22

Mouse anti Human CD88 antibody, clone P12/1

货号:MCA2059T,MCA2059

规格:25 µg,0.2 mg

价格:935,3162

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD88

物种:人

宿主:小鼠

抗体亚型:IgG2a

克隆号:P12/1

荧光染料:Purified

产品介绍
Mouse anti Human CD88 antibody, clone P12/1recognizes the C5a receptor (C5aR) also known as CD88 or C5a anaphylatoxin chemotactic receptor 1. CD88 is predominantly expressed on cells of the myeloid lineage.When C5aR is preincubated with C5a, Mouse anti Human CD88 antibody, clone P12/1 does not bind to the receptor, as the binding site of P12/1 is located in the C5a binding region (Werfelet al. 1996andWeinmanet al. 2003)
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide
ImmunogenC5aR - peptide: Met1- Asn31
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse X63 - Ag8 myeloma cell line.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/50
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Werfel, T.et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.J Immunol. 157: 1729-35.2.Opperman, M.et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and anatagonize C5a biological activity.In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 955-6.3.Pollok-Kopp Bet al.(2007) Dynamics of protein kinase C-mediated phosphorylation of the complement C5a receptor on serine 334.J Biol Chem. 282 (7): 4345-53.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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