Bio-Rad MCA1424F CD21抗体,mouse anti bovine CD21:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1424F CD21抗体,mouse anti bovine CD21:FITC

2024-10-22

mouse anti bovine CD21:FITC

货号：MCA1424F

规格：0.1 mg

价格：3783

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD21

物种：Bovine

宿主：小鼠

抗体亚型：IgG1

克隆号：CC21

荧光染料：FITC

产品介绍
Mouse anti Bovine CD21 monoclonal antibody, clone CC21recognizes the bovine CD21 cell surface antigen, a ~145 kDa single pass type I membrane glycoprotein containing multiplesushidomains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessenset al.1990). CD21 may be expressed on B cells as either a long or a short form (Pringleet al.2012)Mouse anti bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 withPrPcon B cells of scrapie infected sheep (Hallidayet al.2005).
产品详情
Target Species	Bovine
Species Cross-Reactivity	Target Species	Cross Reactivity
Goat	√
Sheep	√
Red deer	√
Mule deer	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 1 x106cells in 100ul.
数据
Figure A. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse anti Bovine CD21 (MCA1424F). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests/1ml
文献
1. Gondaira, S.et al.(2020) Immunosuppression in Cows following Intramammary Infusion ofMycoplasma bovis.Infect Immun. 88 (3) Feb 20 [Epub ahead of print].2. Khosa, S.et al.(2020) Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations.Viruses. 12 (12) Dec 12 [Epub ahead of print].3. Ramm, R.et al.(2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovinein vivomodel.Xenotransplantation. 27 (2): e12571.4. Ramos, A.et al.(2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.Vet Immunol Immunopathol. 206: 1-8.5. Okino, C.H.et al.(2020) A polymorphic CD4 epitope related to increased susceptibility toBabesia bovis.in Canchim calves.Vet Immunol Immunopathol. 230: 110132.6. Park, D.S.et al.(2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.Cell Stress Chaperones. 26 (4): 705-20.7. Radley, G.et al.(2020)In Vitro.Benchmarking Study of Ventricular Assist Devices in Current Clinical Use.J Card Fail. 26 (1): 70-79.8. Colombatti Olivieri, M.A.et al.(2021) Evaluation of a virulent strain ofMycobacterium avium.subsp.paratuberculosis.used as a heat-killed vaccine.Vaccine. NNov 10;S0264-410X(21)01433-X.9. Casaro, S.et al.(2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytesVet Immunol Immunopathol. 248: 110417.

技术参数

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Bio-Rad MCA1424F CD21抗体,mouse anti bovine CD21:FITC

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