Bio-Rad 人 MCA1268F CD39抗体,Mouse anti Human CD39:FITC

2024-10-22

Mouse anti Human CD39:FITC

货号:MCA1268F

规格:0.1 mg

价格:2865

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD39

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:A1

荧光染料:FITC

产品介绍
Mouse anti Human CD39, clone A1recognizes the human CD39 cell surface antigen, a ~70-100 kDa molecule expressed on peripheral blood B cells, T cells and monocytes, and weakly expressed by granulocytes.CD39 has intrinsic ecto-ATPase activity (Wanget al.1996), and expression can be induced on T cells and increased on B cells, as a late activation antigen (Maliszewskiet al.1994).Mouse anti Human CD39, clone A1 has been shown to block MHC independent target cell recognition by hapten-specific CTL (Stocklet al.2001).
产品详情

Target Species

Human

Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

1% Bovine Serum Albumin

ImmunogenPHA activated human lymphocytes
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

FITC

490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:FITCMCA928FF100 Tests
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数据

Figure A. PE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse anti Human CD39 (MCA1268F). All experiments performed on human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
文献
1. Stöckl, J.et al.(2001) Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL.J Immunol. 167 (5): 2724-33.2. Scholzen, A.et al.(2009) Plasmodium falciparum-mediated induction of human CD25Foxp3 CD4 T cells is independent of direct TCR stimulation and requires IL-2, IL-10 and TGFbeta.PLoS Pathog. 5: e1000543.3. Borsellino, G.et al.(2007) Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppressionBlood. 110:1225-32.4. Mittag, D.et al.(2010) The effector T cell response to ryegrass pollen is counterregulated by simultaneous induction of regulatory T cells.J Immunol. 184: 4708-16.5. Loeuillet, C.et al.(2008) In vitro whole-genome analysis identifies a susceptibility locus for HIV-1.PLoS Biol. 6: e32.6. Rawstron, A.C.et al.(2010) Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing.Cytometry B Clin Cytom. 78 Suppl 1: S42-6.7. Alam, M.S.et al.(2009) CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice.J Infect Dis. 199: 494-504.8. Moreno-Fernandez, M.E.et al.(2011) Regulatory T cells control HIV replication in activated T cells through a cAMP-dependent mechanism.Blood. 117: 5372-80.9. Guevara-Flores, A.et al.(2008) 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.Mol Biochem Parasitol. 162: 123-33.10.Glenn, J.R.et al.(2008) Raised levels of CD39 in leucocytosis result in marked inhibition of ADP-induced platelet aggregation via rapid ADP hydrolysis.Platelets. 19: 59-69.11. Häusler SFet al.(2014) Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion.Am J Transl Res. 6 (2): 129-39.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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