Bio-Rad MCA1218GA CD14抗体,mouse anti PIG CD14-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1218GA CD14抗体,mouse anti PIG CD14

2024-10-22

mouse anti PIG CD14

货号：MCA1218GA

规格：0.1 mg

价格：2185

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD14

物种：pig

宿主：小鼠

抗体亚型：IgG2b

克隆号：MIL2

荧光染料：Purified

产品介绍
Mouse anti Pig CD14, clone MIL2recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001) . Mouse anti Pig CD14, clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thackeret al.2001) .Mouse anti pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickovaet al.2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersenet al.2007,Sanzet al.2007).
产品详情
Target Species	Pig
Product Form	Purified IgG - liquid
Species Cross-Reactivity	Target Species	Cross Reactivity
Human	√
N.B. Antibody reactivity and working conditions may vary between species.
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Porcine peripheral blood lymphocytes.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized Balb/c mice were fused with cells from the P2-X63-Ag.653 mouse myeloma.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence	√
Immunohistology - Frozen	√
Flow Cytometry	√	1/25	1/200
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2b Negative Control	MCA691	F	100 Tests
数据
Staining of pig peripheral blood granulocytes with Mouse anti Pig CD14 antibody, clone MIL2 (MCA1218GA) followed by FITC conjugated Goat anti Mouse IgG antibody (STAR117F)
文献
1. Uitterdijk, A.et al.(2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.PLoS One. 12 (6): e0178779.2. Sánchez, E.G.et al.(2017) Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production.Sci Rep. 7 (1): 10369.3. Fernández-Caballero, T.et al.(2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.Dev Comp Immunol. 81: 95-104.4. Sautter, C.A.et al.(2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.Dev Comp Immunol. 84: 181-92.5. López, E.et al.(2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.BMC Vet Res. 15 (1): 91.6. Forner, R.et al.(2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.PLoS One. 16 (5): e0249366.7. Skovdal, S.M.et al.(2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.PLoS One. 14 (10): e0223647.8. Vreman, S.et al.(2018) Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation.Dev Comp Immunol. 84: 361-70.9. Lau, C.et al.(2020) NHDL, a recombinant VL/VHhybrid antibody control for IgG2/4 antibodies.MAbs. 12 (1): 1686319.10. Nielsen, O.L.et al.(2021) A porcine model of subcutaneousStaphylococcus aureus.infection: a pilot study.APMIS. Mar 01 [Epub ahead of print].

技术参数

产品应用 IHC;Flow;IF

产品类型 Monoclonal Antibody

推荐用于 IHC;Flow;IF

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Bio-Rad MCA1218GA CD14抗体,mouse anti PIG CD14

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