Bio-Rad MCA1218F CD14抗体,Mouse anti Pig CD14:FITC

2024-10-22

Mouse anti Pig CD14:FITC

货号:MCA1218F

规格:0.1 mg

价格:3783

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD14

物种:pig

宿主:小鼠

抗体亚型:IgG2b

克隆号:MIL2

荧光染料:FITC

产品介绍
Mouse anti Pig CD14, clone MIL2recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001) . Mouse anti Pig CD14, clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thackeret al.2001) .Mouse anti pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickovaet al.2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersenet al.2007,Sanzet al.2007).
产品详情

Target Species

Pig

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Species Cross-Reactivity

Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

1% Bovine Serum Albumin

Carrier FreeYes

Immunogen

Porcine peripheral blood lymphocytes.

Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized Balb/c mice were fused with cells from the P2-X63-Ag.653 mouse myeloma.

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG2b Negative Control:FITCMCA691FF100 Tests
数据

Figure A. FITC conjugated Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218F) and A647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218F) and A647 conjugated Mouse anti Porcine CD45 antibody, clone K252.1E4 (MCA1222A647). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
文献
1. Uitterdijk, A.et al.(2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.PLoS One. 12 (6): e0178779.2. Sánchez, E.G.et al.(2017) Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production.Sci Rep. 7 (1): 10369.3. Fernández-Caballero, T.et al.(2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.Dev Comp Immunol. 81: 95-104.4. Sautter, C.A.et al.(2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.Dev Comp Immunol. 84: 181-92.5. López, E.et al.(2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.BMC Vet Res. 15 (1): 91.6. Forner, R.et al.(2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.PLoS One. 16 (5): e0249366.7. Skovdal, S.M.et al.(2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.PLoS One. 14 (10): e0223647.8. Vreman, S.et al.(2018) Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation.Dev Comp Immunol. 84: 361-70.9. Lau, C.et al.(2020) NHDL, a recombinant VL/VHhybrid antibody control for IgG2/4 antibodies.MAbs. 12 (1): 1686319.10. Nielsen, O.L.et al.(2021) A porcine model of subcutaneousStaphylococcus aureus.infection: a pilot study.APMIS. Mar 01 [Epub ahead of print].

技术参数

产品应用 IHC(F);Flow;IF

产品类型 Monoclonal Antibody

推荐用于 IHC(F);Flow;IF

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