MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), FITC, eBioscience™

货号： 11-5320-82,11-5320-80

规格：100ug,25 ug

价格：1649,615

产品类型：流式抗体

品牌：eBioscience

抗原：MHC Class II I-Ab

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：AF6-120.1

荧光染料：FITC

类型:	一抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™(#11-4724-81)
浓度：	0.5 mg/mL	用法：	1.0µg/test（Flow）

产品详细信息Description: This AF6-120.1.2 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4.The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed.Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息I-Ab (H2-Ab1, histocompatibility 2, class II antigen A, beta 1) is an MHC class II heterodimer molecule of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-bindingsite blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II. H2-Ab1 heterodimer chains alpha (DQA) and beta (DQB) are both anchored in the membrane. H2-Ab1 plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for polymorphisms is routinely done for bone marrow transplantation.仅用于科研。不用于诊断过程。

数据

MHC Class II I-Ab Antibody (11-5320-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.5 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or 0.5 µg of Anti-Mouse MHC Class II I-Ab FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Frontiers in immunologyMutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction."Published figure using MHC Class II I-Ab monoclonal antibody (Product # 11-5320-82) in Flow Cytometry"2. Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells."Published figure using MHC Class II I-Ab monoclonal antibody (Product # 11-5320-82) in Flow Cytometry"3. The Journal of clinical investigationGuanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis."11532082 was used in flow cytometry to show that deletion of the gene encoding RAB guanine nucleotide exchange factor 1 in keratinocytes severely impairs epidermal barrier function in mice"AuthorsMarichal T,Gaudenzio N,El Abbas S,Sibilano R,Zurek O,Starkl P,Reber LL,Pirottin D,Kim J,Chambon P,Roers A,Antoine N,Kawakami Y,Kawakami T,Bureau F,Tam SY,Tsai M,Galli SJ4. Journal for immunotherapy of cancerCombined immune checkpoint protein blockade and low dose whole body irradiation as immunotherapy for myeloma."11532082 was used in flow cytometry to test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lymphodepleting whole body radiation model"AuthorsJing W,Gershan JA,Weber J,Tlomak D,McOlash L,Sabatos-Peyton C,Johnson BDYear2015SpeciesMouse 5. Proceedings of the National Academy of Sciences of the United States of AmericaReduced production of B-1-specified common lymphoid progenitors results in diminished potential of adult marrow to generate B-1 cells.AuthorsBarber CL,Montecino-Rodriguez E,Dorshkind K

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