Mouse anti Human CD32 antibody, clone AT10,Mouse anti Human CD32 antibody, clone AT10

2024-10-22

Mouse anti Human CD32 antibody, clone AT10

货号:MCA1075EL

规格:0.5 mg

价格:5041

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD32

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:AT10

荧光染料:Low Endotoxin

产品介绍
Mouse anti Human CD32 antibody, clone AT10recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larssonet al.1997).
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present
ImmunogenK562 cell line.
Approx. Protein ConcentrationsIgG concentration 1 mg/ml
Endotoxin Level< 0.01 EU/ug
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry20ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
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文献
1.Greenman, J.et al.(1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.Mol Immunol. 28 (11): 1243-54.

技术参数

产品应用 Flow;IHC;IP

产品类型 单克隆抗体

推荐用于 Flow;IHC;IP

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