mouse anti human AGGRECAN 伯乐 MCA1454G AGGRECAN抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human AGGRECAN 伯乐 MCA1454G AGGRECAN抗体

2024-10-29

mouse anti human AGGRECAN

货号：MCA1454G

规格：0.2 mg

市场价格：3315

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：AGGRECAN

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：7D4

荧光染料：Purified

产品介绍
Mouse anti Human Aggrecan antibody, clone 7D4recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).Using a panel of core protein-directed antibodies directed against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. highlighting a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintinoet al.2009). Mouse anti Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, identified as a cluster 1 isoform.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Bovine	√
Chicken	X
Fish	X
Rat	X
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Purified human articular cartilage aggrecan.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Immunocytochemistry	√
Immunohistology - Frozen	√
Immunoprecipitation	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Betre, H.et al.(2002) A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineeringEngineering in Medicine and Biology 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference,: 829-30.2. Shintani Net al.(2013) TGF-β1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy.PLoS One. 8 (1): e53086.3. Blosa, M.et al.(2013) Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body--implications for physiological functions.Neuroscience. 228: 215-34.4. Wan, S.et al.(2016) Self-assembling peptide hydrogel for intervertebral disc tissue engineering.Acta Biomater. 46: 29-40.5. Rogers, S.L.et al.(2018) Normal Development of the Perineuronal Net in Humans; In Patients with and without Epilepsy.Neuroscience. 384: 350-60.6. Goldberg-Bockhorn, E.et al.(2018) Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering.Lasers Med Sci. 33 (2): 375-84.7. De Moor, L.et al.(2020) Scaffold Free Microtissue Formation for Enhanced Cartilage Repair.Ann Biomed Eng. 48 (1): 298-311.

技术参数

产品应用 IHC;ELISA;WB;IP

产品类型 Monoclonal Antibody

推荐用于 IHC;ELISA;WB;IP

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mouse anti human AGGRECAN 伯乐 MCA1454G AGGRECAN抗体

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