Bio-Rad MCA1948F CD49c抗体,mouse anti human CD49c:FITC

2024-10-22

mouse anti human CD49c:FITC

货号:MCA1948F

规格:0.1 mg

价格:2865

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD49c

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:17C6

荧光染料:FITC

产品介绍
Mouse anti Human CD49c antibody, clone 17C6recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin

Immunogen

CD49c transfected cell line.

Approx. Protein ConcentrationsIgG concentration 0.1mg/ml
Fusion PartnersCells from immunised Balb/c mice were fused with cells of the NS2 mouse myeloma cell line.

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

FITC

490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 5ul of the suggested working dilution to label 106cells in 100ul.
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文献
1. Nbaum, G.et al.(2006) Peptide p277 of HSP60 signals T cells: inhibition of inflammatory chemotaxis.Int Immunol. 18: 1413-9.2. Steinberg, F.et al.(2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.J Cell Biol. 197 (2): 219-30.3. Zanin-Zhorov, A.et al.(2003) T cells respond to heat shock protein 60 via TLR2: activation of adhesion and inhibition of chemokine receptors.FASEB J. 17: 1567-9.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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