Bio-Rad MCA1033PE CD71抗体,Rat anti Mouse CD71: PE

2024-10-22

Rat anti Mouse CD71: PE

货号:MCA1033PE

规格:100 Tests

价格:3162

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD71

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a

克隆号:YTA74.4

荧光染料:PE

产品介绍
Rat anti Mouse CD71 antibody, clone YTA74.4recognizes the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron.Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridgeet al.1982).
产品详情
Target SpeciesMouse
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
ReconstitutionReconstitute with 1 ml distilled water
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide1%Bovine Serum Albumin
ImmunogenConcanavilin A activated mouse spleen cells.
Approx. Protein ConcentrationsIgG concentration 1 mg/ml
Fusion PartnersSpleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
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DescriptionProduct CodeApplicationsPack Size
Rat IgG2a Negative Control:FITCMCA1212FF100 Tests
文献
1.Millot, S.et al.(2010) Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation.Blood. 116: 6072-81.2.Kuo, Y.M.et al.(2004) Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse.Gut. 53: 201-6.3.Krysiak, K.et al.(2014) Reduced Levels of Hspa9 Attenuates Stat5 Activation in Mouse B-cells.Exp Hematol. pii: S0301-472X(14)00817-0.4.Byun, M.et al.(2007) Cowpox virus exploits the endoplasmic reticulum retention pathway to inhibit MHC class I transport to the cell surface.Cell Host Microbe. 2: 306-15.4.Ripich, T. and Jessberger, R. (2011) SWAP-70 regulates erythropoiesis by controlling α4 integrin.Haematologica. 96: 1743-52.

技术参数

产品应用 Flow

产品类型 单克隆抗体

推荐用于 Flow

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