Phire Hot Start II PCR Master Mix

货号：F125L,F125S

规格：1,000 reactions,200 reactions

价格：9313,2354

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Superior yields in significantly shorter timeA 1.5kb fragment from the human cathepsin K gene was amplified with five different hot start DNA polymerases. Phire Hot Start II DNA Polymerase amplified high amounts of specific PCR product in just 29minutes. In contrast, the PCR protocols for hot startTaqDNA polymerases from four major suppliers (A through D) were substantially longer and resulted in lower product yields.描述Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction.Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms everyTaq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared toTaqDNA polymerase.Highlights•Quick hot start—No reactivation step•Fast enzyme—Amplify four times faster than with hot startTaq•Robust—Minimal reaction optimization due to high inhibitor tolerance•High yields—Abundant products due to high efficiency•Longer PCR products—Amplify significantly longer DNA fragments than with any hot startTaqApplications• Hot Start PCR• Routine PCR• Non-high fidelity PCR• Fast PCR• High throughput PCR• GenotypingNote:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that ofTaq-based polymerases. For optimal results start by accurately calculating your Tm with ourTm calculator.Phire Hot Start II amplifies longer fragments than any hot startTaqFive genomic DNA fragments of different lengths were amplified with three different hot start DNA polymerases. Phire Hot Start II DNA Polymerase produced all five amplicons with very high yields. The competing hot startTaqDNA polymerases produced significantly lower yields and failed to amplify the 7.5kb fragment.1 – 0.6kb fragment of human glutathione peroxidase 3 gene2 – 1.0kb fragment of human glutathione peroxidase 3 gene3 – 1.5kb fragment of human cathepsin K gene4 – 2.7 kb fragment of human ß-2-microglobulin gene5 – 7.5 kbfragment of human ß-globin geneComplete PCR protocols in less than half the timeA 600bp fragment from human genomic DNA was amplified with five different hot start DNA polymerases. With Phire Hot Start II DNA Polymerase, the PCR protocol was completed up to four times faster than withTaqDNA polymerases utilizing chemically modified or antibody-based hot start technologies (suppliers A through D). Green buffer further reduces experimental time by eliminating one pipetting step and allowing for direct loading on gel.

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