Bio-Rad Neurofilament H抗体 AHP2259GA,Rabbit anti Bovine Neurofilament H 200kDa

2024-10-22

Rabbit anti Bovine Neurofilament H 200kDa

货号:AHP2259GA

规格:0.1 mg

价格:6528

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Neurofilament H

物种:Bovine

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍

Rabbit anti Bovine Neurofilament H 200 kDa antibodyrecognizes bovine intermediate 200 kDa Neurofilament H (NFH), a 1026 amino acid ~200 kDa protein involved in the maintenance of neuronal integrity.Neurofilaments are a major component of the cellular cytoskeleton, acting as the most abundant support for the axon cytoplasm. They exist as three neurofilament proteins: 68/70 kDa (NFL) 160 kDa (NFM) and 200 kDa (NFH), which are expressed in both the central and peripheral nervous systems. Although typically expressed by neurons, neurofilaments are also expressed by neuroblastomas, neuromas, gangliogliomas, ganglioneuromas, and ganglioneuroblastomas, as well as paragangliomas, carcinoids, skin neuroendocrine carcinomas, and oat cell carcinomas of the lung (Lehtoet al.1983).Defects in NFH are responsible for susceptibility to the neurodegenerative disorder amyotrophic lateral sclerosis (ALS) which affects upper and lower motor neurons, and results in fatal paralysis (Mendonçaet al.2005).

产品详情

Target Species

Bovine

Species Cross-Reactivity

Target Species Cross Reactivity

Rat √

Human √

Mouse √

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

AntiserumPreparation

Antiserum to bovine NFH was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

TRIS-glycine buffered saline, NaCl

Preservative Stabilisers

0.05% Sodium Azide (NaN3)

Immunogen

GST-tagged bovine NFH recombinant protein.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

Immunohistology - Paraffin1

1.0ug/ml

Western Blotting

0.1ug/ml

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Histology Positive Control Tissue
  • Bovine or rat cerebellum
  • Western Blotting
  • AHP2259GA detects a band of approximately 200kDa in bovine cerebellum tissue lysates.

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文献

References for Neurofilament H antibody1.Kaneko, S.et al.(2006) Protecting axonal degeneration by increasing nicotinamide adenine dinucleotide levels in experimental autoimmune encephalomyelitis models.J Neurosci. 26: 9794-804.2.Li, Y.et al.(2008) Transplanted olfactory ensheathing cells incorporated into the optic nerve head ensheathe retinal ganglion cell axons: possible relevance to glaucoma.Neurosci Lett. 440: 251-4.3.Ignatov, A.et al.(2003) Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development.J Neurosci. 23: 907-14.4.Leypoldt, F.et al.(2002) Neuronal differentiation of cultured human NTERA-2cl.D1 cells leads to increased expression of synapsins.Neurosci Lett. 324 (1): 37-40.5.Savolainen, H. (2009) Cortical demyelination in PML and MS: similarities and differences.Neurology. 72 (9): 861.6.Zhang, Z.et al.(2011) The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration.Biomaterials. 32: 4109-17.7.Chen, K.et al.(2016) Differential Histopathological and Behavioral Outcomes Eight Weeks after Rat Spinal Cord Injury by Contusion, Dislocation, and Distraction Mechanisms.J Neurotrauma. 33 (18): 1667-84.Further Reading1.Liu Qet al.(2004) Neurofilament proteins in neurodegenerative diseases.Cell Mol Life Sci. 61 (24): 3057-75.

技术参数

产品应用 P*; WB

产品类型 Polyclonal Antibody

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