IL-1 beta Human ELISA Kit ELISA试剂盒,IL-1 beta Human ELISA Kit/人IL-1 β ELISA试剂盒

2024-10-23

IL-1 beta Human ELISA Kit/人IL-1 β ELISA试剂盒

货号:KHC0011,KHC0012,KHC0011C

规格:96 Tests,2X96 Tests,5X96 Tests

价格:7303,11232,24645

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1 pg/mL

检测范围3.9-250 pg/mL

样本类型/体积

上清液 50 μL

血清 50μL

操作时间

1 hr 20 min

测试时间

2 hr 55 min

Homogenous (no wash)

No

批间变异系数(CV)6.6%
批内变异系数(CV)4.4%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5X96 Tests

组份

- 预包被96孔板

- 标准品

- 标准稀释液

- 生物素化检测抗体

- 链霉亲和素-辣根过氧化物酶

- 辣根过氧化物酶稀释液

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameIL-1 beta
Protein aliasesCatabolin, IL-1 beta, interleukin 1, beta, Interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIL-1, IL1-BETA, IL1B, IL1F2
Gene ID(Human) 3553
Gene symbolIL1B
UniProt ID

(Human) P01584

The Human Interleukin-1beta (Hu IL-1beta) ELISA quantitates Hu IL-1β in human serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1β.人白介素-1β(人IL-1β)ELISA定量测定人血清,缓冲液或细胞培养基中的人IL-1β。 该检测试剂盒将专门识别天然和重组的人IL-1β。Principle of the method 方法原理The Human IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-1β固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.白介素-1 β(IL-1 β)是单核细胞,巨噬细胞和树突状细胞表达的促炎细胞因子。响应炎症刺激,IL-1β合成为31 kDa的无活性前体,并累积在细胞质中。将前IL-1β切割成活性的17 kDa蛋白需要激活炎性小体,炎性小体是对病原体,应激条件和其他危险信号有反应的多蛋白复合物。炎性体激活触发半胱天冬酶-1前体加工成其活性形式,进而切割前IL-1 β。 IL-1β缺乏经典ER /高尔基体途径的信号序列肽,并通过一种另类和不完全了解的机制与半胱天冬酶-1一起分泌。尽管IL-1β最常以其活性形式分泌,但在某些生物学条件下可检测到未切割蛋白的分泌。 IL-1 β通过两个受体IL-1RI和IL-1RII发出信号,这两个受体均与IL-1 α共享。 IL-1β活性可以由IL-1受体拮抗剂(IL-1RA)调节,IL-1RA是许多细胞类型产生的蛋白质,通过竞争性抑制作用阻断受体结合。 IL-1β通过触发靶细胞中其他促炎细胞因子的产生并启动对感染和损伤的急性期反应,在先天宿主防御中发挥重要作用。 IL-1β水平升高与许多慢性炎症疾病相关,IL-1β中和抗体具有潜在的治疗价值。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-1 beta Human ELISA KitRepresentative standard curve for Human IL-1 beta ELISA

技术参数
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