IL-4 Human ELISA Kit ELISA试剂盒,IL-4 Human ELISA Kit/人IL-4 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-4 Human ELISA Kit ELISA试剂盒,IL-4 Human ELISA Kit/人IL-4 ELISA试剂盒

2024-10-23

IL-4 Human ELISA Kit/人IL-4 ELISA试剂盒

货号：KHC0041

规格：96 tests

价格：7227

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<2 pg/mL
检测范围	7.8-500 pg/mL
样本类型/体积	上清液 100 μL 血清 100μL 血浆 100μL
操作时间	1 hr 20 min
测试时间	3 hr
Homogenous (no wash)	No
批间变异系数（CV）	4.5%
批内变异系数（CV）	3%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 辣根过氧化物酶稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-4
Protein aliases	B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, Binetrakin, BSF-1, IL-4, interleukin 4 variant 2, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL4
Gene ID	(Human) 3565
Gene symbol	IL4
UniProt ID	(Human) P05112

The Human Interleukin 4 (Hu IL-4) ELISA quantitates Hu IL-4 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-4.人白介素4（人IL-4）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人IL-4。该检测试剂盒将只识别天然和重组的人IL-4。Principle of the method 方法原理The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-4固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.白介素-4（IL-4）是由活化的T细胞产生的多效性免疫调节细胞因子，可作为白介素4受体的配体。已经发现IL-4具有最广泛的作用范围。白介素4受体也与IL13结合，有助于该细胞因子和IL13的许多重叠功能。 STAT6是一种信号转导和转录激活因子，已显示在介导IL-4的免疫调节信号中起重要作用。 IL-4基因，IL-3，IL-5，IL-13和CSF2在5q号染色体上形成细胞因子基因簇。特别地，IL-4在染色体上紧邻IL13。此外，发现IL-4基因，IL13和IL5由染色体上120kb范围内的几个远程调控元件协调。 IL-4δ2是IL-4的已知剪接变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-4 Human ELISA KitRepresentative standard curve for Human IL-4 ELISA and for Human IL-4 ultrasensitive ELISA

技术参数

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IL-4 Human ELISA Kit ELISA试剂盒,IL-4 Human ELISA Kit/人IL-4 ELISA试剂盒

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