SMAD2 Polyclonal Antibody/SMAD2多克隆抗体

货号：51-1300

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：A 27 amino acid synthetic peptide derived from the

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.25 mg/mL	用法：	1-3 µg x 10^6 cells(ChIP)；0.1-1.0 µg/mL(ELISA)；1:20-1:200(IHC (P))；1-3 µg/mL(WB)

靶标信息SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

数据：

SMAD2 Antibody (51-1300) in IHC (P)Immunohistochemistry analysis of SMAD2 showing staining in the nucleus of paraffin-embedded human breast tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SMAD2 polyclonal antibody (Product # 51-1300) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. SMAD2 Antibody (51-1300)The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2014 -Figure 6. Mixed Smad complexes are expressed in mammary tumors but not normal mammary epithelium. (A-F) MDA-MB-231 xenograft tumors grown in the mammary fat pads of mice show expression for all three Smad complexes: (D) canonical BMP Smad, (E) canonical TGF-beta Smad and (F) mixed Smad complexes, whereas mammary epithelium, with a more normal histological appearance, shows expression of canonical Smad complexes (A, B), but not mixed Smad complexes (C). (G-L) A human breast tumor shows the expression of canonical and mixed Smads (J-L) whereas normal breast tissue from the same patient expresses the canonical TGF-beta Smad complexes (H) but no mixed Smad complexes (I). Arrowheads mark examples of positive rolling circle amplification (RCA) products in panels with detectable signals. Scale, 10 um.

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参考文献：

1. International journal of stem cellsTGF-βinduces Smad2 Phosphorylation, ARE Induction, and Trophoblast Differentiation.2. The American journal of pathologyKLF10 Gene Expression Modulates Fibrosis in Dystrophic Skeletal Muscle.3. PloS oneMicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2.4. eLifeResponse to Nodal morphogen gradient is determined by the kinetics of target gene induction.5. Nature medicinePreventive and therapeutic effects of Smad7 on radiation-induced oral mucositis.

技术参数

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