TIE2 Human ELISA Kit ELISA试剂盒,TIE2 Human ELISA Kit/人TIE2 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

TIE2 Human ELISA Kit ELISA试剂盒,TIE2 Human ELISA Kit/人TIE2 ELISA试剂盒

2024-10-23

TIE2 Human ELISA Kit/人TIE2 ELISA试剂盒

货号：BMS2042,BMS2042TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.03 ng/mL
检测范围	0.16-10.0 ng/mL
样本类型/体积	血清 10 μL 血浆 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	21 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	6.3%
批内变异系数（CV）	7.1%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 对照 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	TIE2 (TEK)
Protein aliases	Angiopoietin-1 receptor, CD202b, Endothelial tyrosine kinase, hTIE2, p140 TEK, TEK tyrosine kinase, endothelial, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CD202B, TEK, TIE-2, TIE2, VMCM, VMCM1
Gene ID	(Human) 7010
Gene symbol	TEK
UniProt ID	(Human) Q02763

The Human Tyrosine Kinase Receptor (Hu TIE-2) ELISA quantitates Hu TIE-2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIE-2.人酪氨酸激酶受体（人TIE-2）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人TIE-2。该检测试剂盒将专门识别天然和重组的人TIE-2。Principle of the method 方法原理The Human TIE-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TIE-2固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.TIE2，也称为TEK酪氨酸激酶，TIE2基因定位于9p21.2。该基因编码属于蛋白质酪氨酸激酶Tie2家族的受体。编码的蛋白质具有独特的细胞外区域，该区域包含两个免疫球蛋白样结构域，三个表皮生长因子（EGF）样结构域和三个纤连蛋白III型重复序列。配体血管生成素-1与该受体结合并介导在胚胎血管发育中起作用的信号通路。免疫印迹显示，甲状腺刺激激素和增加细胞内cAMP的药物可增加TIE2的表达。表达受体酪氨酸激酶TIE2的HSC是静止的和抗凋亡的，并且包括HSC的侧群，其粘附在骨髓生境中的成骨细胞上。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

TIE2 Human ELISA KitRepresentative standard curve for Human TIE-2 ELISA

技术参数

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TIE2 Human ELISA Kit ELISA试剂盒,TIE2 Human ELISA Kit/人TIE2 ELISA试剂盒

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