sIL-2R/CD25 (Soluble) Human ELISA Kit ELISA试剂盒,sIL-2R/CD25 (Soluble) Human ELISA Kit/人sIL-2R / CD25（可溶）ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

sIL-2R/CD25 (Soluble) Human ELISA Kit ELISA试剂盒,sIL-2R/CD25 (Soluble) Human ELISA Kit/人sIL-2R / CD25（可溶）ELISA试剂盒

2024-10-23

sIL-2R/CD25 (Soluble) Human ELISA Kit/人sIL-2R / CD25（可溶）ELISA试剂盒

货号：BMS212-2,BMS212-2TEN

规格：96 Tests,10X96 Tests

价格：6120,36168

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.27 ng/mL
检测范围	0.31-20.0 ng/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	9.8%
批内变异系数（CV）	7.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	CD25
Protein aliases	CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, interleukin 2 receptor, alpha, Interleukin-2 receptor subunit alpha
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CD25, IDDM10, IL2R, IL2RA, IMD41, p55, TCGFR
Gene ID	(Human) 3559
Gene symbol	IL2RA
UniProt ID	(Human) P01589

The Human Soluble Interleukin 2 Receptor/CD25 (Hu IL-2R) ELISA quantitates Hu IL-2R in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-2R.人可溶性白介素2受体/ CD25（人IL-2R）ELISA定量了人血清，血浆，缓冲溶液或细胞培养基中的人IL-2R。该检测试剂盒将只识别天然和重组的人IL-2R。Principle of the method 方法原理The Human IL-2R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-2R固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.CD25（IL2受体α链/ IL2RA）是一种细胞因子，在T和B淋巴细胞的增殖中发挥作用。该细胞因子的受体（IL2RA）是异源三聚体蛋白复合物，具有由白介素4（IL4）和白介素7（IL7）共享的γ链。 IL2RA，IL2Rβ链（IL2RB）和IL2Rγ链（IL2RG）构成了高亲和力的IL2受体。同型二聚体IL2RA链产生低亲和力受体，同型二聚体IL2RB链产生中等亲和力受体。 IL2在成熟胸腺细胞中的表达是单等位基因，代表了控制单个基因精确表达的异常调节模式。 IL2主要由成熟的T细胞产生。 IL2作为生长因子，分化因子和细胞死亡调节剂起着重要作用。 IL-2刺激B细胞的增殖，增强自然杀伤细胞的活性，并抑制粒细胞巨噬细胞集落的形成。小鼠中类似基因的定向破坏会导致溃疡性结肠炎样疾病，在对抗原刺激的免疫反应中起作用。该基因的突变与白介素2受体α缺乏有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

sIL-2R/CD25 (Soluble) Human ELISA KitRepresentative standard curve for Human IL-2 Receptor ELISA

技术参数

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sIL-2R/CD25 (Soluble) Human ELISA Kit ELISA试剂盒,sIL-2R/CD25 (Soluble) Human ELISA Kit/人sIL-2R / CD25（可溶）ELISA试剂盒

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