HSP90 alpha Human ELISA Kit ELISA试剂盒,HSP90 alpha Human ELISA Kit/人HSP90 α ELISA试剂盒

2024-10-23

HSP90 alpha Human ELISA Kit/人HSP90 α ELISA试剂盒

货号:BMS2090,BMS2090TEN

规格:96 Tests,10X96 Tests

价格:6018,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.03 ng/mL

检测范围0.156-10 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

细胞裂解液 100 μL

操作时间

1 hr 20 min

测试时间

4 hr

Homogenous (no wash)

No

批间变异系数(CV)10%
批内变异系数(CV)8.6%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 对照

- 胶板盖

储存温度

2-8℃

Protein nameHSP90 alpha
Protein aliasesepididymis luminal secretory protein 52, epididymis secretory sperm binding protein Li 65p, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, alpha-like 4, heat shock 90kD protein, heat shock 90kDa protein 1, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein 90kDa alpha family class A member 1, Heat shock protein HSP 90-alpha, HSP 86, LAP-2, Lipopolysaccharide-associated protein 2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesEL52, HEL-S-65p, HSP86, Hsp89, HSP89A, Hsp90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP-2, LAP2
Gene ID(Human) 3320
Gene symbolHSP90AA1
UniProt ID(Human) P07900
The Human Heat Shock Protein 90 alpha (Hu HSP90 alpha) ELISA quantitates Hu HSP90 alpha in human serum, plasma cell lysate. The assay will exclusively recognize both natural and recombinant Hu HSP90 alpha.人热休克蛋白90α(人HSP90 α)ELISA定量了人血清,浆细胞裂解物中的人HSP90 α。 该检测试剂盒将专门识别天然和重组人HSP90α。Principle of the method 方法原理The Human HSP90 alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人HSP90 alpha固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.热激蛋白(HSP)是响应各种生物应激(包括热)表达的蛋白。 HSP90是在压力条件下诱导的90 kDa蛋白,但还是在非压力条件下发现的最丰富的细胞蛋白之一。 已经发现HSP90与许多其他细胞内蛋白相关,包括类固醇受体,肌动蛋白,微管蛋白和一些激酶。 已经提出,HSP90对于维持至少一些类固醇受体的结构完整性是必需的,以允许在受体活化期间结合配体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

HSP90 alpha Human ELISA KitRepresentative standard curve for Human HSP90 alpha ELISA

技术参数
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