Human Recombinant BDNF/人重组BDNF
货号：78005,78005.1,78005.3,78005.2
规格：10 μg,100 μg,500 μg,1000 μg
价格：1908,13176,37776,57776
产品类型：细胞因子添加物
品牌：STEMCELL Technologies
| Brain-derived neurotrophic factor (BDNF), like nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4), is a member of the NGF family of neurotrophins, which are required for the differentiation and survival of specific neuronal subpopulations in both the central and the peripheral nervous systems (Minichiello & Klein; Minichiello et al.). BDNF binds with high affinity to the tropomyosin receptor kinase B (TrkB), and activates AKT and ERK pathways (Mattson et al.). It is expressed in the hippocampus, cortex, and synapses of the basal forebrain. BDNF acts as a survival factor for human embryonic stem cells when plated on either feeder cells or Corning® Matrigel® (Pyle et al.). BDNF regulates synaptic transmission and plasticity at adult synapses in the central nervous system, and contributes to adaptive neuronal responses including long-term potentiation, long-term depression, certain forms of short-term synaptic plasticity, and homeostatic regulation of neuronal excitability (Reichardt). It also has a role in neurogenesis by promoting survival and growth of dorsal root ganglion cells, and hippocampal and cortical neurons (Binder & Scharfman). BDNF, together with Human Recombinant GDNF (glial cell line-derived neurotrophic factor; Catalog #78058), BrainPhys™ Neuronal Medium (Catalog #05790), and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.). |
数据： |
| （A）通过促进C6细胞增殖测定人重组BDNF的生物活性。用荧光法测定培养7天后细胞增殖情况。 EC50是细胞增殖在最大值的50%时生长因子的有效浓度。上例中 EC50 为 0.65 μg/mL。（B）在还原和非还原(-）条件下，用SDS-PAGE对1μg人重组BDNF进行了分离，并用考马斯蓝染色进行染色。人重组BDNF为13.6kda亚基的同二聚体，预测总分子质量为27.3kDa。 |
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技术参数 细胞类型 PSC衍生神经细胞; 神经干细胞和祖细胞; 神经元
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