stemline CD34 造血干细胞扩增培养基 无血清,Stemline II Hematopoietic Stem Cell Expansion Medium 无血清 造血干细胞扩增培养基

2024-10-23

Stemline II Hematopoietic Stem Cell Expansion Medium 无血清 造血干细胞扩增培养基

货号:S0192

规格:500ml

价格:1900

产品类型:造血干细胞培养

品牌:Sigma

1. 成份明确、无血清培养基;

2. 灵活性(培养基中未添加任何细胞因子);

3. Stemline多余1600篇HSC研究领域的文献;

3. Stemline II-与合适的添加物配合使用,HSC扩增能力更强;

4. Stemline II造血干细胞扩增培养基-世界上第一个用于培养HSC的GMP级培养基。

Stemline II是第二代Sigma造血干细胞扩增培养基家族成员,旨在优化平衡分化细胞和未分化细胞,同时最大化其细胞扩增效率。 Stemline II可与来自骨髓、脐带血和动员外周血的造血干细胞(HSC)相兼容,其已被证明可显著增加所有三种来源的细胞的扩增。 临床规模扩增细胞后通过流式细胞术分析,在扩增短期植入所需的CD34+/CD38+晚期祖细胞实验中,Stemline II表现出比其它市售培养基更高的扩增效率。 在Stemline培养基中扩增的人脐带血细胞移植到免疫缺陷的NOD/SCID小鼠中后,表现出令人印象深刻的自我更新能力,其说明了Stemline在真实功能临床试验中的有效性。

Stemline II 造血干细胞扩增培养基不含血清和所有其它动物源性成分。这些成分的排除提高了该培养基性能的一致性,并且消除了与潜在偶然因素相关的安全风险。

Stemline造血干细胞扩增培养基在GMP最先进的设施中进行生产,是您HSC应用的绝佳选择。如果您正在考虑在研究性新药研发中申请使用该产品,请联系我们:010-57438396; Email: Info@nwbiotec.com。

Expansion of CD34+ cells from cord blood in

Stemline™ and Stemline™ II compared to other

commercially available, serum-free, HSC media.

Assays were set up in 24-well tissue culture plates

with each medium tested in triplicate. 1 ml of the

appropriate medium was added to each well, and

the plate was inoculated with 10,000 cells/well.

The cells were counted on day 10 and the fold

increase was determined by cells final/cells inital.

In cord blood, the Stemline™ media outperform

the other serum-free HSC media. While Stemline™

already performed better than or equal to the

other HSC media, Stemline™ II exhibited

approximately a 3 fold increase in expansion

over Stemline™.

Stemline demonstrates superior expansion of cord blood hematopoietic stem cells (HSC). To test the ability of Stemline II Hematopoietic Stem Cell Expansion Medium to expand CD34+ HSCs, researchers at Sigma Aldrich and the University of Kentucky designed a bench-scale expansion assay. Cells were seeded into the wells of 24-well tissue culture plates. One milliliter of medium was added to each well with the appropriate cytokines to stimulate growth (100 ng/ml each of TPO, SCF, and G-CSF). Each condition was performed in triplicate and seeded with 10,000 cells per ml in each well. Due to the clinical importance and the donor-to-donor variability typical to the expansion of umbilical cord blood-derived cells, researchers elected to test 15 donors for expansion and surface antigen expression. The cells were counted on day 10 and the fold increase was determined by cellsfinal/cellsinitial. HSCs from cord blood cultured in Stemline and Stemline II demonstrated superior expansion to those grown in other serum-free HSC media.

Stemline demonstrates superior expansion of bone marrow hematopoietic stem cells (HSC). To test the ability of Stemline II Hematopoietic Stem Cell Expansion Medium to expand CD34+ HSCs, researchers at Sigma Aldrich and the University of Kentucky designed a bench-scale expansion assay. Cells were seeded into the wells of 24-well tissue culture plates. One milliliter of medium was added to each well with the appropriate cytokines to stimulate growth (100 ng/ml each of TPO, SCF, and G-CSF). Each condition was performed in triplicate and seeded with 10,000 cells per ml in each well. The cells were counted on day 14 and the fold increase was determined by cellsfinal/cellsinitial. HSCs from bone marrow cultured in Stemline and Stemline II demonstrated superior expansion to those grown in other serum-free HSC media.

相关产品:

人脐血CD34+造血干细胞

技术参数

产品优点 1. 成份明确、无血清培养基;2. 灵活性(培养基中未添加任何细胞因子);3. Stemline:1600多篇HSC研究领域的文献发表,行业领先;3. Stemline II-与合适的添加物配合使用,HSC扩增能力更强;4. Stemline II造血干细胞扩增培养基-世界上第一个用于培养HSC的GMP级培养基。

产品应用 造血干细胞扩增PBMC扩增

细胞类型 造血干细胞

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