SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)/SMAD2重组兔单克隆抗体（31H15L4）

货号：700048

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：A peptide corresponding to amino acids 81-107 of Q

物种：人

宿主：兔

抗体亚型：IgG

克隆号：31H15L4

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	3 µg(ChIP)；1-5 µg/mL(ELISA)；1-3 µg/1x10^6 cells(Flow)；1 µg/mL(ICC)；1 µg/mL(IF)；0.5-1 µg/mL(WB)

产品详细信息This antibody is predicted to react with bovine, carp, chicken, goldfish, mouse, opossum, orangutan, rat, Xenopus and zebrafish based on sequence homology.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

数据：

SMAD2 Antibody (700048) in IFImmunofluorescence analysis of SMAD2 was done on 70% confluent log phase TGF- beta treated HeLa cells (serum starved for 16 hours followed by treatment with 20 ng/mL TGF-beta for 1 hour). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD2 Recombinant Rabbit Monoclonal Antibody (Product # 700048) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is an untreated HeLa cells showing cytoplasmic localization. Panel e shows nuclear localization of SMAD2 upon treatment. Panel f is no primary antibody control. The images were captured at 20X magnification. SMAD2 Antibody (700048) in FlowFlow cytometry analysis of SMAD2 in Jurkat cells using a SMAD2 recombinant rabbit monoclonal antibody (Product # 700048) at a dilution of 0.5 µg. Cells were fixed and permeabilized using FIX and PERM (Product # GAS-004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (black) compared to an isotype control (gray).

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参考文献：

1. Developmental cellLong-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages.2. Molecular endocrinology (Baltimore, Md.)FGFR2IIIb-MAPK Activity Is Required for Epithelial Cell Fate Decision in the Lower Müllerian Duct.3. Molecular endocrinology (Baltimore, Md.)FGFR2IIIb-MAPK Activity Is Required for Epithelial Cell Fate Decision in the Lower Müllerian Duct.

技术参数

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