Rat anti Mouse CD45:StarBright Blue 700

货号：MCA1031SBB700

规格：100 Tests/0.5ml

价格：4148

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD45

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b

克隆号：YW62.3

荧光染料：StarBright Blue 700

类型:	单抗	同型对照：	IgG2b
浓度：		用法：	5 µL/test(Flow)
免疫原性：	Mouse spleen cells.

Rat anti Mouse CD45 antibody, clone YW62.3recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes.CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komuraet al.1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shenet al.1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5bencoding Ly5.2 and the allele in SJL mice becoming Ly5aencoding Ly5.1 (Morseet al.1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45agene was renamed Ptprcaand CD45brenamed Ptprcb(Charbonneauet al.1988;Zebedeeet al.1991).A number of different isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkelandet al.1989).Rat anti mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45.N.B. Some reactivity with human tissue has been observed.

数据

Figure A. StarBright Violet 440 conjugated Rat anti Mouse CD3 (MCA500SBV440). Figure B StarBright Violet 440 conjugated Rat anti Mouse CD3 (MCA500SBV440) and StarBright Blue 700 conjugated Rat and Mouse CD45 (MCA1031SBB700). All experiments performed on red cell lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer

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参考文献：

1. Watt, S.M.et al.(1987) Cell-surface markers on haemopoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations.Mol Cell Probes. 1 (4): 297-326.2. Zirger, J.M.et al.(2012) Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells.Mol Ther. 20 (4): 808-19.3. Long, G.G.et al.(2010) Hematopoietic Proliferative Lesions in the Spleen of rasH2 Transgenic Mice Treated with MNU.Toxicol Pathol. 38: 1026-36.4. Drake, C.et al.(2011) Brain inflammation is induced by co-morbidities and risk factors for stroke.Brain Behav Immun. 25: 1113-22.5. Chan, D.A.et al(2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.Cancer Cell. 15: 527-38.6. Lebson, L.et al.(2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.J Neurosci. 30: 9651-8.7. Lee, D.C.et al.(2010) LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice.J Neuroinflammation. 7: 56.8. Wang, S.et al.(2008) Drak2 contributes to West Nile virus entry into the brain and lethal encephalitis.J Immunol. 181: 2084-91.9. Paz, H.et al.(2010) The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.Blood. 116: 1254-62.10. Reed-Geaghan, E.G.et al.(2010) Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.J Neurosci. 30: 15369-73.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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