| 产品详细信息Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.Applications Reported: BM8 has been reported for use in flow cytometric analysis.Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. |
| 1.OncoimmunologyAdipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer."11-4801 was used in Flow cytometry/Cell sorting to compare the expression of PD-L1 in mature adipocytes versus preadipocytes."AuthorsWu B,Sun X,Gupta HB,Yuan B,Li J,Ge F,Chiang HC,Zhang X,Zhang C,Zhang D,Yang J,Hu Y,Curiel TJ,Li R2.OncoimmunologyTim-3 promotes tumor-promoting M2 macrophage polarization by binding to STAT1 and suppressing the STAT1-miR-155 signaling axis."11-4801 was used in Flow cytometry/Cell sorting to demonstrate that Tim-3 polarizes macrophages by directly binding to STAT1 via residue Y256 and Y263 in its intracellular tail and inhibiting the STAT1-miR-155-SOCS1 signaling axis."AuthorsJiang X,Zhou T,Xiao Y,Yu J,Dou S,Chen G,Wang R,Xiao H,Hou C,Wang W,Shi Q,Feng J,Ma Y,Shen B,Li Y,Han G3.OncoimmunologyTim-3 promotes tumor-promoting M2 macrophage polarization by binding to STAT1 and suppressing the STAT1-miR-155 signaling axis."11-4801 was used in Flow cytometry/Cell sorting to demonstrate that Tim-3 polarizes macrophages by directly binding to STAT1 via residue Y256 and Y263 in its intracellular tail and inhibiting the STAT1-miR-155-SOCS1 signaling axis."AuthorsJiang X,Zhou T,Xiao Y,Yu J,Dou S,Chen G,Wang R,Xiao H,Hou C,Wang W,Shi Q,Feng J,Ma Y,Shen B,Li Y,Han G4.Frontiers in immunologyLenalidomide and Programmed Death-1 Blockade Synergistically Enhances the Effects of Dendritic Cell Vaccination in a Model of Murine Myeloma."11-4801 was used in Flow cytometry/Cell sorting to investigate the efficacy of dendritic cell vaccination in combination with lenalidomide and PD-1 blockade in a model of murine myeloma."AuthorsVo MC,Jung SH,Chu TH,Lee HJ,Lakshmi TJ,Park HS,Kim HJ,Rhee JH,Lee JJ5.NaturePluripotency factors functionally premark cell-type-restricted enhancers in ES cells."11-4801 was used in Immunocytochemistry-Immunofluorescence to examine the hierarchical and obligate premarking of cell-type-restricted enhancers in embryonic stem cells."AuthorsKim HS,Tan Y,Ma W,Merkurjev D,Destici E,Ma Q,Suter T,Ohgi K,Friedman M,Skowronska-Krawczyk D,Rosenfeld MG |
QQ:1749072012