| 抗体类型 | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| 浓度： | 0.2 mg/mL | 用法： | 0.125 µg/test (Flow) |
| 产品详细信息Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: The M1/70 antibody has been reported for use in flow cytometric analysis.Applications Tested: The M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes or bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus. |
| 数据 |
| CD11b Antibody (12-0112-82) in FlowStaining of mouse bone marrow cells with 0.06 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD11b PE (purple histogram). Cells in the large scatter population were used for analysis. |
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| 参考文献： |
| 1.Frontiers in immunologyTpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells."Published figure using CD11b monoclonal antibody (Product # 12-0112-82) in Flow Cytometry"2.NPJ vaccinesNeutrophil swarming and extracellular trap formation play a significant role in Alum adjuvant activity."Published figure using CD11b monoclonal antibody (Product # 12-0112-82) in Flow Cytometry"3.Frontiers in molecular neuroscienceIncreased White Matter Inflammation in Aging- and Alzheimer's Disease Brain."12-0112 was used in Flow cytometry/Cell sorting to indicate that microglia-induced neuroinflammation is predominant in the white matter of aging mice and humans as well as in EOAD brains."AuthorsRaj D,Yin Z,Breur M,Doorduin J,Holtman IR,Olah M,Mantingh-Otter IJ,Van Dam D,De Deyn PP,den Dunnen W,Eggen BJL,Amor S,Boddeke E4.EMBO molecular medicineVEGF blockade enhances the antitumor effect of BRAFV600E inhibition."12011282 was used in immunohistochemistry - frozen section to show that although targeted inhibition of either BRAF or VEGF initially suppresses the growth of BRAF-mutant tumors, combined inhibition of both pathways results in apoptosis and long-lasting tumor responses"AuthorsComunanza V,Corà D,Orso F,Consonni FM,Middonti E,Di Nicolantonio F,Buzdin A,Sica A,Medico E,Sangiolo D,Taverna D,Bussolino F5.PloS oneNeutrophils contribute to excess serum BAFF levels and promote CD4+ T cell and B cell responses in lupus-prone mice."12-0112 was used in Flow cytometry/Cell sorting to analyse the phenotype, localisation and function of neutrophils in the spleens of healthy mice and congenic lupus-prone mice."AuthorsCoquery CM,Wade NS,Loo WM,Kinchen JM,Cox KM,Jiang C,Tung KS,Erickson LD |
QQ:1749072012