Phire Green Hot Start II PCR Master Mix

货号：F126L,F126S

规格：1,000 reactions,200 reactions

价格：9145,2280

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction. The master mix also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared toTaqDNA polymerase.Highlights•Quick hot start—No reactivation step•Fast enzyme—Amplify four times faster than with hot startTaq•Robust—Minimal reaction optimization due to high inhibitor tolerance•High yields—Abundant products due to high efficiency•Longer PCR products—Amplify significantly longer DNA fragments than with any hot startTaq•Direct loading on gelApplications• Hot Start PCR• Routine PCR• Non-high fidelity PCR• Fast PCR• High throughput PCR• GenotypingNote:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that ofTaq-based polymerases. For optimal results start by accurately calculating your Tm with ourTm calculator.Phire Hot Start II amplifies longer fragments than any hot startTaqFive genomic DNA fragments of different lengths were amplified with three different hot start DNA polymerases. Phire Hot Start II DNA Polymerase produced all five amplicons with very high yields. The competing hot startTaqDNA polymerases produced significantly lower yields and failed to amplify the 7.5kb fragment.1 – 0.6kb fragment of human glutathione peroxidase 3 gene2 – 1.0kb fragment of human glutathione peroxidase 3 gene3 – 1.5kb fragment of human cathepsin K gene4 – 2.7 kb fragment of human ß-2-microglobulin gene5 – 7.5 kbfragment of human ß-globin geneEfficient amplification of fragments up to 7.5 kb with master mix formatFive genomic DNA fragments of different lengths were amplified with different hot start PCR master mixes. Phire Green Hot Start II PCR Master Mix produced all five amplicons with high yields. Other hot start PCR master mixes produced lower or no yields, with some also amplifying non-specific products.1 – 0.47 kb fragment of human calpain 10 gene2 – 1.1 kb fragment of human KLK3 gene3 – 1.7 kb fragment of human PPAR gamma gene4 – 3.5 kb fragment of human plasminogen activator gene5 – 7.5 kb fragment of human hemoglobin B geneSuperior yields in short time with master mix formatA 2 kb fragment of human β-globin gene was amplified with different hot start PCR master mixes. Phire Green Hot Start II PCR Master Mix delivered high yields of specific product in just 41 minute whereas all other master mixes provided lower yields and in most cases required longer protocols.

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