Thermo CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), FITC,CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), FITC,CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), FITC

2024-10-24

CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), FITC

货号：11-2231-80,11-2231-82

规格：25μg,100μg

价格：727,1674

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

荧光染料：FITC

抗体类型	单抗	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), FITC
浓度：	0.5 mg/mL	用法：	0.1 μg/test (Flow)
产品详细信息Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragmentcontaining all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223.CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
数据
CD223 (LAG-3) Antibody (11-2231-82) in FlowStaining of 3-day Anti-Mouse CD3e and CD28 Functional Grade Purified (Product # 16-0031-82andProduct # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.5 µg of Rat IgG1 K Isotype Control FITC (Product # 11-4301-82) (blue histogram) or 0.5 µg of Anti-Mouse CD223 (Lag-3) FITC (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
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参考文献：
1.Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy."Published figure using CD223 (LAG-3) monoclonal antibody (Product # 11-2231-82) in Flow Cytometry"2.CellEpithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments."Published figure using CD223 (LAG-3) monoclonal antibody (Product # 11-2231-82) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency."Published figure using CD223 (LAG-3) monoclonal antibody (Product # 11-2231-82) in Flow Cytometry"

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