| 产品详细信息Description: The RMT3-23 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. The RMT3-23 antibody reacts with CD366 protein expressed by both BALB/c and C57BL/6 strains of mice. CD366, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. CD366 is expressed selectively by differentiated CD4^+Th1 and CD8^+Tc1 cells, but is absent on CD4^+Th2 and CD8^+Tc2 cells. Other hematopoietic cell types, including naive T cells, B cells, macrophages and dendritic cells, do not express CD366, at least at the protein level. CD366 expression is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, CD366 was shown to be expressed on most CD4^+ and CD8^+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4^+ cells in the periphery expressed CD366 after immunization.RMT3-23 has been shown to have functional activity; blocks DC recognition of apoptotic cells and also induces autoantibody production.Applications Reported: The RMT3-23 antibody has been reported for use in flow cytometric analysis.Applications Tested: The RMT3-23 antibody has been tested by flow cytometric analysis of mouse CD366 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice. |
| 1.OncoimmunologyGrowth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression."12-5870 was used in Flow cytometry/Cell sorting to show that bone morphogenetic protein-4 causes immunosuppression by augmenting PD-L1 expression in the mesenchymal subset of lung cancer cells."AuthorsChen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL2.Frontiers in microbiologyDown-Regulation of Tim-3 in Monocytes and Macrophages inPlasmodiumInfection and Its Association with Parasite Clearance."Published figure using CD366 (TIM3) monoclonal antibody (Product # 12-5870-82) in Flow Cytometry"3.OncoimmunologyAngiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype."12-5870 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype."AuthorsShrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CW4.Molecular metabolismTim-3 aggravates podocyte injury in diabetic nephropathy by promoting macrophage activation via the NF-κB/TNF-α pathway."12-5870 was used in Flow cytometry/Cell sorting to study T cell immunoglobulin domain and mucin domain-3 as a regulator in renal inflammation."AuthorsYang H,Xie T,Li D,Du X,Wang T,Li C,Song X,Xu L,Yi F,Liang X,Gao L,Yang X,Ma C5.Cell reportsPulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer."12-5870 was used in Flow cytometry/Cell sorting to set the foundation and show the importance of a combinatorial therapeutic strategy using pulsatile targeted therapy together with immunotherapy to optimally enhance tumor delay and promote long-term anti-tumor immunity."AuthorsChoi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD |
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