Thermo CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 710,CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 7-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 710,CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 7

2024-10-24

CD335 (NKp46) Monoclonal Antibody (29A1.4), PerCP-eFluor 710

货号：46-3351-80,46-3351-82

规格：25 μg,100 μg

价格：1164,3402

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：PerCP-eFluor™ 710

抗体类型	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor 710
浓度：	0.2 mg/mL	用法：	0.25 μg/test (Flow)
产品详细信息Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).
数据
CD335 (NKp46) Antibody (46-3351-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 0.125 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.125 µg of Anti-Mouse CD335 (NKp46) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.
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参考文献：
1.Wellcome open researchConventional NK cells and ILC1 are partially ablated in the livers of Ncr1iCreTbx21fl/flmice.2.Nature communicationsLoss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis."Published figure using CD335 (NKp46) monoclonal antibody (Product # 46-3351-82) in Flow Cytometry"3.The Journal of allergy and clinical immunologyInnate lymphoid cells contribute to allergic airway disease exacerbation by obesity."46-3351 was used in Flow cytometry/Cell sorting to indicate that high-fat diet-induced obesity might exacerbate allergic airway inflammation through mechanisms involving ILC2s and ILC3s."AuthorsEveraere L,Ait-Yahia S,Molendi-Coste O,Vorng H,Quemener S,LeVu P,Fleury S,Bouchaert E,Fan Y,Duez C,de Nadai P,Staels B,Dombrowicz D,Tsicopoulos A4.Cellular and molecular immunologyThe breast tumor microenvironment alters the phenotype and function of natural killer cells."Published figure using CD335 (NKp46) monoclonal antibody (Product # 46-3351-82) in Flow Cytometry"5.The Journal of allergy and clinical immunologyInnate lymphoid cells contribute to allergic airway disease exacerbation by obesity."46-3351 was used in Flow cytometry/Cell sorting to indicate that high-fat diet-induced obesity might exacerbate allergic airway inflammation through mechanisms involving ILC2s and ILC3s."AuthorsEveraere L,Ait-Yahia S,Molendi-Coste O,Vorng H,Quemener S,LeVu P,Fleury S,Bouchaert E,Fan Y,Duez C,de Nadai P,Staels B,Dombrowicz D,Tsicopoulos A

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