Thermo CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7,CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7,CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7

2024-10-24

CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7

货号：25-2001-80,25-2001-82

规格：25μg,100μg

价格：1578,3861

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：PE-Cy7

抗体类型	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7
浓度：	0.2 mg/mL	用法：	0.25 μg/test (Flow)
产品详细信息Description: This Ba13 monoclonal antibody recognizes mouse CD200R3, a surface receptor expressed by basophils and mast cells.Applications Reported: This Ba13 antibody has been reported for use in flow cytometric analysis.Applications Tested: This Ba13 antibody has been tested by flow cytometric analysis of the MC/9 cell line. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser靶标信息CD200R3 is a dimeric cell surface receptor also known as CD200RLb. CD200R3 contains two Ig-like extracellular domains and as such is a member of the immunoglobulin superfamily of receptors. The intracellular region of CD200R3 associates with the adaptor molecule DAP12, and possibly also DAP10, CD3 zeta and FcR gamma-chain, indicating that it is capable of transducing stimulatory signals. In this respect CD200R3 bears similarity to activatory receptors CD200R2 and CD200R4. In contrast, an inhibitory receptor CD200R (also known as CD200R1) relies on other adaptors. CD200R3 is primarily expressed by basophils and mast cells, cells known to be involved in anti-parasitic immune responses and frequently also allergic reactions. The surface expression of CD200R3 decreases when basophils are activated by ligating their CD16 and CD32. Remarkably, most of this downregulation is observed within the first 30 minutes after activation. Unlike CD200R, CD200R3 does not bind CD200.
数据
CD200R3 Antibody (25-2001-82) in FlowMC/9 cells were stained with 0.125 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4321-82) (blue histogram) or 0.125 µg of CD200R3 Monoclonal Antibody, PE-Cyanine7 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
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Thermo CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7,CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7

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