CD103 (Integrin alpha E) Monoclonal Antibody (Ber-ACT8), FITC, eBioscience

货号：11-1037-41

规格：25 Tests

市场价格：1029

产品类型：流式抗体

品牌：eBioscience

抗原：CD103

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：Ber-ACT8

荧光染料：FITC

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The Ber-ACT8 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen (E-cadherin) binds to CD103 and mediateshoming of lymphocytes to the intestinal epithelium. Cross-blocking studies suggest that B-Ly7 and Ber-ACT8 see different epitopes.Applications Reported: This Ber-ACT8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This Ber-ACT8 antibody has been pre-titrated and tested by flow cytometric analysis of 2-3 day PHA-activated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteinscomposed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.仅用于科研。不用于诊断过程。

数据

CD103 (Integrin alpha E) Antibody (11-1037-41) in FlowStaining of 3-day cultured (left) or 3-day PHA-stimulated (right) human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and Anti-Human CD103 (Integrin alpha E) FITC. Total viable cells were used for analysis.

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