Mouse anti Human CD49f antibody 伯乐 MCA1457GA CD49f抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human CD49f antibody 伯乐 MCA1457GA CD49f抗体

2024-10-29

Mouse anti Human CD49f antibody

货号：MCA1457GA,MCA1457,MCA1457T

规格：0.1 mg,0.2 mg,25 µg

市场价格：2023,3783,935

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD49f

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：450-30A

荧光染料：Purified

产品介绍
Mouse anti Human CD49f antibody, clone 450-30Arecognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f.CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes.CD49f is also widely expressed on epithelial tissues.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide
Carrier Free	Yes
Immunogen	alpha 6 beta 4 integrin purified from A431 cells.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow	√	1/10	1/50
Immunohistology - Frozen1	√	1/50	1/100
Immunohistology - Paraffin	x
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 10⁶cells in 100ul. Histology Positive Control TissueTonsil
数据
Western blot analysis of Jurkat whole cell lysate probed with Mouse anti Human CD49f antibody, clone 450-30A (MCA1457GA) followed by HRP conjugated Goat anti Human IgG, visualized using chemiluminescence.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
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文献
1. Cavers, M.et al.(2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.Clin Exp Immunol. 127: 60-5.2. López, J.et al.(2012) Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance.BMC Cancer. 12: 48.3. Keller, P.J.et al.(2012) Defining the cellular precursors to human breast cancer.Proc Natl Acad Sci U S A. 109: 2772-7.4. Kaczmarek, M.et al.(2011) Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions.Tumour Biol. 32: 1123-32.5. Aldridge, V.et al.(2012) Human mesenchymal stem cells are recruited to injured liver in a β1-integrin and CD44 dependent manner.Hepatology. 56 (3): 1063-73.6. Steinberg, F.et al.(2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.J Cell Biol. 197 (2): 219-30.7. Liu, L.et al.(2003) Priming of eosinophil migration across lung epithelial cell monolayers and upregulation of CD11b/CD18 are elicited by extracellular Ca2+.Am J Respir Cell Mol Biol. 28: 713-21.8. Goyer, B.et al.(2017) Extracellular matrix and integrin expression profiles in Fuchs endothelial corneal dystrophy cells and tissue model.Tissue Eng Part A. Jul 20 [Epub ahead of print].

技术参数

产品应用 C* ；F ；WB

产品类型单克隆抗体

推荐用于 C* ；F ；WB

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Mouse anti Human CD49f antibody 伯乐 MCA1457GA CD49f抗体

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