IgM Monoclonal Antibody (II/41), PerCP-eFluor 710,

货号：46-5790-80,46-5790-82

规格：25 ug,100 ug

市场价格：1211,3603

产品类型：流式抗体

品牌：eBioscience

抗原：lgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：PerCP-eFluor™ 710

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor 710
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: This II/41 antibody has been reported for use inflow cytometric analysis.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (46-5790-80) in FlowStaining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.25 µg of Anti-Mouse IgM PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)Cellular Dynamics of Memory B Cell Populations: IgM+ and IgG+ Memory B Cells Persist Indefinitely as Quiescent Cells.2. Cell reportsChronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.3. Disease models & mechanismsA mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).4. Genes & developmentConversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.5. AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

技术参数

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