mouse anti human CD326:Alexa Fluor® 647 伯乐MCA1870A647 CD326抗体

2024-10-29

mouse anti human CD326:Alexa Fluor® 647

货号:MCA1870A647

规格:100 Tests/1ml

市场价格:2567

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD326

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:VU-1D9

荧光染料:Alexa Fluor 647

产品介绍
Mouse anti Human CD326 antibody, clone VU-1D9recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, andde novoexpression being seen in dysplastic squamous epithelium.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor 647 - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin

ImmunogenHG9 cell line (small cell lung carcinoma)
Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Fusion PartnersSpleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®647650665

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
数据

Figure A. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control(MCA928A647). Figure B. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse anti Human CD326 (MCA1870A647). All experiments performed on HT-29 cells gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928AF647Flow100 Tests
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文献
References for CD326 antibody1. Koga, Y.et al.(2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.Gastroenterol Res Pract. 2008: 605273.2. Fillmore, C.M.et al.(2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.Proc Natl Acad Sci U S A. 107 (50): 21737-42.3. Murakata, A.et al.(2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.Ann Surg. 253: 94-100.4. Keller, P.J.et al.(2012) Defining the cellular precursors to human breast cancer.Proc Natl Acad Sci U S A. 109 (8): 2772-7.5. Jeong, H.T.et al.(2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.Liver Int. 32: 430-40.6. Armstrong, A.J.et al.(2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.PLoS One. 14 (5): e0216934.Further Reading7. Winter, M.J.et al.(2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.Am J Pathol. 163 (6): 2139-48.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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