| 产品介绍 | | |
| Mouse anti Human C3a Receptor antibody, clone hC3aRZ1recognizes the receptor for human complement component C3a (C3aR), a ~55 kDa cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes.Expression levels of C3aR on human cells varies significantly between donors. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to Alexa Fluor®488 - liquid |
| Reconstitution | Reconstitute with 1 ml distilled water |
| Preparation | - MCA2455A488: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA2455A488T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
|
| Fusion Partners | Spleen cells from immunised rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line. |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Recombinant human C3aR protein. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide1%Bovine Serum Albumin |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Werfel, T.et al.(2000) Activated human T lymphocytes express a functional C3a receptor.J Immunol. 165 (11): 6599-605.2.Soruri, A.et al.(2003) IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migrationin vivo.J Immunol. 170 (6): 3306-14.3.Zwirner, J.et al.(1999) Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies.Immunology. 97: 166-72.4.Kacani, L.et al.(2001) C5a and C5a(desArg) enhance the susceptibility of monocyte-derived macrophages to HIV infection.J Immunol. 166: 3410-5.5.Raby, A.C.et al.(2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.J Immunol. 183: 506-17. |
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