TNF alpha Monoclonal Antibody (MP6-XT22), PE, eBioscience™

货号：12-7321-82,12-7321-81

规格：100 µg,50 µg

价格：2486,1237

产品类型：流式抗体

品牌：eBioscience

抗原：TNF alpha

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：MP6-XT22

荧光染料：PE

类型:	一抗	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.Applications Reported: This MP6-XT22 antibody has been reported for use in intracellular staining followed by flowcytometric analysis.Applications Tested: This MP6-XT22 antibody is tested by intracellular flow cytometric analysis of stimulated splenocytes. It is offered in 2 formats: - µg size: has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.仅用于科研。不用于诊断过程。

数据

TNF alpha Antibody (12-7321-82) in FlowMouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) or 0.125 µg of Anti-Mouse TNF alpha PE. Viable cells as determined Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) in the lymphocyte gate were used for analysis.

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参考文献：

1. Frontiers in immunologyExpression of Siglec-E Alters the Proteome of Lipopolysaccharide (LPS)-Activated Macrophages but Does Not Affect LPS-Driven Cytokine Production or Toll-Like Receptor 4 Endocytosis."12-7321 was used in Flow cytometry/Cell sorting to reveal that induction of siglec-E by LPS can modulate the phenotype of macrophages, the functional significance of which is currently unclear."AuthorsNagala M,McKenzie E,Richards H,Sharma R,Thomson S,Mastroeni P,Crocker PRYear2019SpeciesMouse 2. Frontiers in immunologyCD4 T Helper Cells Instruct Lymphopenia-Induced Memory-Like CD8 T Cells for Control of Acute LCMV Infection."12-7321 was used in Flow cytometry/Cell sorting to determine whether memory-like T cells can efficiently respond to foreign antigen and participate in antiviral immunity."AuthorsSchmitt ME,Sitte S,Voehringer DYear2019SpeciesMouse 3. Cell reportsType I Interferon Delivery by iPSC-Derived Myeloid Cells Elicits Antitumor Immunity via XCR1+Dendritic Cells."12-7321 was used in Flow cytometry/Cell sorting to suggest that IFN-α-iPSC-pMCs might constitute effective immune-stimulating agents for cancer that are refractory to checkpoint blockade."AuthorsTsuchiya N,Zhang R,Iwama T,Ueda N,Liu T,Tatsumi M,Sasaki Y,Shimoda R,Osako Y,Sawada Y,Kubo Y,Miyashita A,Fukushima S,Cheng Z,Nakaki R,Takubo K,Okada S,Kaneko S,Ihn H,Kaisho T,Nishimura Y,Senju S,Endo I,Nakatsura T,Uemura YYear2019SpeciesMouse 4. CellAdaptive Immune Resistance Emerges from Tumor-Initiating Stem Cells."12-7321 was used in Flow cytometry/Cell sorting to place tSCs at the crux of how immune checkpoint pathways are activated."AuthorsMiao Y,Yang H,Levorse J,Yuan S,Polak L,Sribour M,Singh B,Rosenblum MD,Fuchs EYear2019SpeciesMouseDilution1:100 5. Cell reportsAutophagy Regulation of Metabolism Is Required for CD8+T Cell Anti-tumor Immunity."12-7321 was used in Flow cytometry/Cell sorting to demonstrate a cell-autonomous role for autophagy in the regulation of CD8+ T-cell-mediated control of tumors."AuthorsDeVorkin L,Pavey N,Carleton G,Comber A,Ho C,Lim J,McNamara E,Huang H,Kim P,Zacharias LG,Mizushima N,Saitoh T,Akira S,Beckham W,Lorzadeh A,Moksa M,Cao Q,Murthy A,Hirst M,DeBerardinis RJ,Lum JJ

技术参数

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