Lynx Rapid Plus DyLight®550 antibody conjugation kit™ LNK231D550 bio-rad ,Lynx Rapid Plus DyLight®550 antibody conjugation-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Lynx Rapid Plus DyLight®550 antibody conjugation kit™ LNK231D550 bio-rad ,Lynx Rapid Plus DyLight®550 antibody conjugation

2024-10-22

Lynx Rapid Plus DyLight®550 antibody conjugation kit™

货号：LNK231D550,LNK233D550

规格：3 Conjugations For 200µg Antibody,3 Conjugations For 20µg Antibody

价格：3910,6843

产品类型：流式抗体

品牌：Bio-Rad

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：DyLight 550

产品介绍
Lynx Rapid Plus DyLight 550 antibody conjugation kit™enables the ultra rapid conjugation of a pre-prepared lyophilized mixture containing DyLight 550 label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in the coupling of DyLight 550 to the antibody.The LYNX Rapid Plus kit can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
产品详情
Reagents in the Kit	3 Vials LYNXTMRapid Plus lyophilized DyLight®550 mix1 Vial LYNXTMRapid Plus Modifier reagent1 Vial LYNXTMRapid Plus Quencher reagent
Preparing The Antibody	Pack Size: 3 Conjugations For 200µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5.Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNXTMchemicals, and Thiomersal should also be avoided. Azide (0.02-0.1%), BSA (0.1-0.5%), EDTA, Glycerol (up to 50%), and common non-buffering salts and sugars have little or no effect on conjugation efficiency.It is recommended that 100-200ug antibody be used in each labelling reaction. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 100ul and a recommended antibody amount of 100ug. A maximum of 200ug of antibody can be used to obtain good quality conjugates as long as the maximum conjugation volume of 100ul is not exceeded. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit.Pack Size: 3 Conjugations For 20µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5.Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNXTMchemicals, and Thiomersal should also be avoided. Azide (0.02-0.1%), BSA (0.1-0.5%), EDTA, Glycerol (up to 50%), and common non-buffering salts and sugars have little or no effect on conjugation efficiency.It is recommended that 10-20ug antibody be used in each labelling reaction. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 10ul and a recommended antibody amount of 10ug. A maximum of 20ug of antibody can be used to obtain good quality conjugates as long as the maximum conjugation volume of 10ul is not exceeded. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This kit contains lyophilized hygroscopic components that are moisture-sensitive. This kit is shipped under ambient conditions with silica packets to avoid exposure to moisture. On receipt, Bio-Rad recommend that the kit is stored at -20℃ and protected from moisture. Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing. Before opening, allow the components to reach room temperature to minimize condensation.
应用
Application Name	Verified	Min Dilution	Max Dilution
Conjugation	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Instructions For Use 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25°C) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.

技术参数

产品类型 Conjugation Kit

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Lynx Rapid Plus DyLight®550 antibody conjugation kit™ LNK231D550 bio-rad ,Lynx Rapid Plus DyLight®550 antibody conjugation

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