Bio-Rad AHP1831B BDNF,Rabbit anti Human BDNF:Biotin

2024-10-22

Rabbit anti Human BDNF:Biotin

货号:AHP1831B

规格:50 µg

价格:4208

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:BDNF

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Biotin

产品介绍
Rabbit anti Human BDNF antibodyrecognizes human brain-derived neurotrophic factor (BDNF), also known as abrineurin. BDNF is a secreted 119 amino acid ~30 kDa protein with an additional 18 amino acid signal peptide and a 110 amino acid propeptide region. BDNF is a potent neurotrophin and member of the NGF-beta family, secreted from dense core vesicles, which promotes the growth, differentiation and survival of neurons within, or directly connected to, the central nervous system (CNS).BDNF signals through binding to the high affinity receptor trkB, and also binds to the low affinity neurotrophin receptor LNGFR. BDNF is involved in the modulation of short-term synaptic function and in the formation of long-term memory (Alonsoet al.2002). BDNF is modulated during progression of Alzheimer's with an early increase and reducel levels in patients with latter stages of Alzheimer's (Laskeet al.2005). Decreased levels of BDNF are also noted in the progression of Huntington's disease (Zuccatoet al.2008) .
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Biotin - lyophilized

Antiserum Preparation

Antiserum to human BDNF were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Approx. Protein Concentrations

IgG concentration 0.1mg/ml after reconstitution

Immunogen

Recombinant human BDNF.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Reconstitution

Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.25

1.0ug/ml

Western Blotting

0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • ELISA
  • This biotinylated human BDNF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with ourpurified human BDNF antibody(AHP1831) as the capture reagent andrecombinant human BDNF(PHP213) as the standard.

技术参数

产品应用 ELISA;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;WB

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