Rabbit anti Human CD254 antibody AHP1209B bio-rad,Rabbit anti Human CD254 antibody

2024-10-22

Rabbit anti Human CD254 antibody

货号:AHP1209B

规格:50 µg

价格:4208

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CD254

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Biotin

产品介绍
Rabbit anti Human interleukin-32 antibodyrecognises IL-32, a ~26 kDa cytokine, thought to play a role in innate and adaptive immune responses. IL-32 is produced by mitogen-activated lymphocytes, interferon-gamma activated epithelial cells and IL-12, IL-18, IL-32-activated NK cells. It is expressed at elevated levels after the activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-kappa-B and p38 MAPK cytokine signal pathways. TNF-alpha, IL-1beta, IL-6 and 2 C-X-C chemokine family members, all involved in autoimmune and inflammatory diseases, including rheumatoid arthritis are induced by IL-32.
产品详情
Target SpeciesHuman
ReconstitutionReconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum PreparationAntisera to human CD254 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Product FormPurified IgG conjugated to Biotin - lyophilized
Buffer SolutionPhosphate buffered saline
ReconstitutionReconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
Carrier FreeYes
ImmunogenRecombinant human CD254.
Approx. Protein ConcentrationsNone present
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Boyle, W.J.et al.(2003) Osteoclast differentiation and activation.Nature. 423 (6937): 337-42.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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