Bio-Rad AAM75 M-CSF,Goat anti Mouse M-CSF antibody

2024-10-22

Goat anti Mouse M-CSF antibody

货号:AAM75

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原: M-CSF

物种:小鼠

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍
Goat anti Mouse M-CSF antibodyrecognizes mouse M-CSF (Macrophage colony-stimulating factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling.
产品详情

Target Species

Mouse

Product Form

Purified IgG - lyophilized

Antiserum Preparation

Antiserum to mouse M-CSF was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Approx. Protein Concentrations

IgG concentration 1.0mg/ml after reconstitution

Immunogen

Recombinant mouse M-CSF.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Reconstitution

Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.5

2.0ug/ml

Functional Assays

0.050.08ug/ml

Western Blotting

0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献
1. Djelloul, M.et al.(2016) RAE-1 expression is induced during experimental autoimmune encephalomyelitis and is correlated with microglia cell proliferation.Brain Behav Immun. 58: 209-17.

技术参数

产品应用 ELISA;FN;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;FN;WB

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